Multimodal single-cell approaches shed light on T cell heterogeneity

Single molecule fluorescence approaches shed light on intracellular RNAs.

Using single-cell multiple omics approaches to resolve tumor heterogeneity

It has become increasingly clear that both normal and cancer tissues are composed of heterogeneous populations. Genetic variation can be attributed to the downstream effects of inherited mutations, environmental factors, or inaccurately resolved errors in transcription and replication. When lesions occur in regions that confer a proliferative advantage, it can support clonal expansion, subclona...

Single cell biotechnology to shed a light on biological ‘dark matter’ in nature

Wei E. Huang,1* Yizhi Song2 and Jian Xu3 Department of Engineering Science, University of Oxford, Parks Road, Oxford OX1 3PJ, UK. Kroto Research Institute, University of Sheffield, Broad Lane, Sheffield S3 7HQ, UK. Single-Cell Center, CAS Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Scienc...

Regulatory T‐cell heterogeneity

Regulatory T cells (Tregs) preserve immune homeostasis by suppressing autoimmunity and inflammation. While most Tregs develop in the thymus, they are heterogenous in their tissue localisation and function. This heterogeneity is advantageous for the maintenance of immune and tissue homeostasis in organs such as gut and kidney but often detrimental in the context of tumor, since Treg cells can da...

Effect of LIGHT Adjuvant on Kinetics of T-Cell Responses Induced by HSV-1 DNA Immunization

Background: Studies on efficacy of various vaccines that prevent or reduce the primary and recurrent HSV-1 infection have demonstrated the importance of cellular immunity for protection against the infection. We previously used DNA vaccination to induce cellular immunity against HSV-1 infection in mice. Objective: The aim of our study was to evaluate the effect of LIGHT, a member of TNF super f...